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A Ventana Benchmark XT automated system (Ventana Medical System, Tucson, AZ, USA) was used to perform IHC on four-micron sections for the rabbit monoclonal antibody GFAP (EP672Y, Cell Marque, CA, USA)
Notes Store in cool place
A chemical reaction in which an atom, molecule, or ion loses one or more electrons
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Add cell lysis buffer (Catalogue No.:E050), collect the lysate solution (total protein concentration measured by BCA assay: 1.66 mg/ml with 20ug total protein loading) to detect.(no obvious band) Lane 2: Wild THP-1 cells were treated with 80nM TPA and cultured overnight, and then stimulated with 100ng/ml LPS for 6 hours, then cultured with 300ng/ml Brefeldin A (BFA) for 3 hours
This means it is hydrating and gentle on skin unlike drying solvent alcohols like denatured alcohol
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